A method for identifying a 5-MeC in a template nucleic is provided. The method comprises providing a template having 5-MeC, converting the 5-MeC into a further modification selected from 5-caC and 5-FC. The converted template is then sequenced, and a change in sequencing is detected that is indicative of the further modification, allowing for identifying the 5-MeC in the template nucleic acid.
Details
Title
Identification of 5-methyl-C in nucleic acid templates
Inventors
Korlach, Jonas : PacBio He, Chuan : University of Chicago Clark, Tyson A. : PacBio Zhang, Liang : University of Chicago Lu, Xingyu : University of Chicago
Applicants
Pacific Biosciences California University of Chicago
Content Type
Patent
Assignees
University of Chicago Pacific Biosciences of California Inc.