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Abstract
Methods and compositions are provided concerning polypeptides with modifications that increase its binding affinity for the Fab region of an antibody. Methods include using the polypeptides for isolating, detecting, purifying, measuring and quantifying Fab polypeptides. Other embodiments concern kits, compositions, and solid supports containing the polypeptides and for using the polypeptides for isolating, detecting, purifying, measuring and quantifying Fab polypeptide.