Single-molecule FRET (smFRET) has become a mainstream technique for studying biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by an ever-increasing number of groups has generated significant progress in sample preparation, measurement procedures, data analysis, algorithms and documentation. Several labs that employ smFRET approaches have joined forces to inform the smFRET community about streamlining how to perform experiments and analyze results for obtaining quantitative information on biomolecular structure and dynamics. The recent efforts include blind tests to assess the accuracy and the precision of smFRET experiments among different labs using various procedures. These multi-lab studies have led to the development of smFRET procedures and documentation, which are important when submitting entries into the archiving system for integrative structure models, PDB-Dev. This position paper describes the current ‘state of the art’ from different perspectives, points to unresolved methodological issues for quantitative structural studies, provides a set of ‘soft recommendations’ about which an emerging consensus exists, and lists openly available resources for newcomers and seasoned practitioners. To make further progress, we strongly encourage ‘open science’ practices.
Details
Title
FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices
Funding Information
National Institutes of Health, GM130942 National Institutes of Health, GM095904 National Institutes of Health, GM079238 National Institutes of Health, GM098859 National Institutes of Health, GM084288 National Institutes of Health, GM137608 National Institutes of Health, GM112882 National Institutes of Health, GM130793 National Institutes of Health, GM140272 National Institutes of Health, GM130375 National Institutes of Health, 128185 National Institute of Diabetes and Digestive and Kidney Diseases National Science Foundation, 1842951 National Science Foundation, 2004016 Human Frontier Science Program, RGP0061/2019 European Research Council, 638536 European Research Council, SMPFv2.0 European Research Council, 671208 European Research Council, 819299 Deutsche Forschungsgemeinschaft, GRK2062 Deutsche Forschungsgemeinschaft, SFB863 Deutsche Forschungsgemeinschaft, PL696/4-1 Deutsche Forschungsgemeinschaft, SPP2191 402723784 Deutsche Forschungsgemeinschaft, SFB 1129 240245660 Deutsche Forschungsgemeinschaft, SE 1195/21-1 Deutsche Forschungsgemeinschaft, SPP 2191 Deutsche Forschungsgemeinschaft, project ID 390939984, CIBSS - EXC-2189 Wellcome Trust, 110164/Z/15/Z Swiss National Science Foundation Biotechnology and Biological Sciences Research Council, BB/S008896/1 Biotechnology and Biological Sciences Research Council, BB/T008032/1 Royal Society, RGS\R2\180405 Royal Society, DKR00620 Royal Society, RGF\R1\180054 Agence Nationale de la Recherche, ANR-17-CE09-0026-02 Agence Nationale de la Recherche, ANR-18-CE11-0004-02 Agence Nationale de la Recherche, ANR-19-CE44-0009-02 Israel Science Foundation, 1250/19 Israel Science Foundation, 3565/20 National Research Foundation of Korea, 2019R1A2C2090896 National Research Foundation of Korea, NRF-2019R1A2C2005209 National Research Foundation of Korea, NRF-2019R1A2C1089808 Independent Fund Denmark, 6110-00623B National Key Research and Development Program of China, 2018YFA0507700 UHasselt, BOF fund, R-10789 Deutsche Forschungsgemeinschaft, SFB1035 Milner Fund KU Leuven, Special Research Fund, C14/16/053 Carlsbergfondet, CF16-0797 Villum Fonden, 18333 Novo Nordisk, NNF14CC0001 Ludwig-Maximilians-Universität München Carolina Cancer Center of Nanotechnology Excellence University of Melbourne Searle Scholars Program University of Zurich EPSRC
Publication Date
2021-03-29
Language
English
Copyright Statement
This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Notes
Due to the large number of authors, only the first 20 and the University of Chicago authors are included on the above author list. Please download the article for the complete list of authors.