Described are peptides for delivery of a nanoparticle to the cytosol, the peptide comprising: (a) a nanoparticle association domain; (b) a proline-rich spacer domain; (c) an uptake domain; and (d) a vesicle escape domain comprising a non-hydrolyzable lipid moiety, wherein the spacer domain is between the nanoparticle association domain and the uptake and vesicle escape domains, and wherein the peptide, when attached to an extracellular nanoparticle, is effective to induce uptake of the nanoparticle by a cell and delivery of the nanoparticle to the cytosol of the cell. Also described are methods of delivery of a nanoparticle to the cytosol of a cell, the method comprising providing to a cell a nanoparticle attached to such a peptide. Exemplary nanoparticles include quantum dots.
Details
Title
Modular functional peptides for the intracellular delivery of nanoparticles
Inventors
Delehanty, James B. : Naval Research Laboratory Medintz, Igor L. : Naval Research Laboratory Mattoussi, Hedi M. : Naval Research Laboratory Deschamps, Jeffrey R. : Naval Research Laboratory Dawson, Glyn : University of Chicago Dawson, Philip E. : University of Chicago Blanco-Canosa, Juan Bautista : Naval Research Laboratory Boeneman, Kelly : Naval Research Laboratory
Applicants
Delehanty, James B. Medintz, Igor L. Mattoussi, Hedi M. Deschamps, Jeffrey R. Dawson, Glyn Dawson, Philip E. Blanco-Canosa, Juan Bautista Boeneman, Kelly US Navy University of Chicago
Content Type
Patent
Assignees
The Government of the United States of America as represented by the Secretary of the Navy The United States of America as represented by the Secretary of the Navy University of Chicago
