Described are nucleic acids encoding a polypeptide for delivery of a nanoparticle to the cytosol, the peptide comprising: (a) a nanoparticle association domain, (b) a spacer domain, (c) an uptake domain, and (d) a vesicle escape domain, wherein the domains (a) through (d) appear in the same order as listed above, and wherein the peptide, upon addition of a non-hydrolyzable lipophilic moiety to the vesicle escape domain and binding to a nanoparticle, is effective to induce uptake of a nanoparticle by a cell and delivery of the nanoparticle to the cytosol of the cell. Also described are methods of delivery of a nanoparticle to the cytosol of a cell, the method comprising providing to a cell a nanoparticle attached to such a peptide. Exemplary nanoparticles include quantum dots.
Details
Title
Modular Functional Peptides for the Intracellular Delivery of Nanoparticles
Inventors
Delehanty, James B. : Naval Research Laboratory Medintz, Igor L. : Naval Research Laboratory Mattoussi, Hedi M. : Naval Research Laboratory Deschamps, Jeffrey R. : Naval Research Laboratory Dawson, Glyn : University of Chicago Dawson, Philip E. : University of Chicago Blanco-Canosa, Juan Bautista : Naval Research Laboratory Boeneman, Kelly : Naval Research Laboratory
Applicants
Delehanty, James B. Medintz, Igor L. Mattoussi Hedi M Deschamps, Jeffrey R. Dawson, Glyn Dawson, Philip E. Blanco-Canosa, Juan Bautista Boeneman, Kelly University of Chicago Government of the USA as represented by the Secretary of the Navy
