This image captures the expression of a GFP-fused calcium sensor in U2OS cell, offering insights into the intricate relationship between calcium bursts and the dynamic behavior of filopodia, driven by MyoX protein expression (not visible here). The fluorescent microscopy image was taken as part of an optimization process for gene transfection, where the sensor was used to track real-time calcium signaling events. The comet-like dotted traces observed here are likely a peculiar result of the staining process, revealing not just a technical artifact but an unexpected visualization of cellular debris. This project, conducted in Ronald Rock's lab, may help elucidate the dependencies between calcium fluxes within the cell and MyoX protein activity, providing a deeper understanding of how calcium dynamics and filopodia extension interplay to regulate cell movement—process that, in worst-case scenarios, contributes to metastasis.