Published December 14, 2023
| Version v1
Journal article
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A Chemical Counterpart to the Resolution Step of Nature's Intein-Mediated Protein Splicing
- 1. University of Chicago
- 2. Rockefeller University
Description
In the course of an attempted total chemical synthesis of the ant insulin-like peptide-2 (ILP2) protein molecule, specific cleavage of a backbone peptide bond in a branched ester-linked polypeptide chain with concomitant peptide splicing was observed. The side reaction was investigated in model compounds. Here, we postulate a chemical mechanism for this novel polypeptide backbone cleavage reaction as a chemical counterpart to the resolution step of biochemical intein-mediated protein splicing.
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Chemical-Counterpart-to-the-Resolution-Step-of-Natures-Intein-Mediated-Protein-Splicing.pdf
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Additional details
Identifiers
- DOI
- 10.1021/acschembio.3c00590
- Other
- oai:uchicago.tind.io:10171
Funding
- Bia-Echo Foundation
- Junior Scholar Award from the Global Consortium for Reproductive Longevity and Equality at the Buck Institute
- Unknown funder
- Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica