Published July 22, 2018
| Version v1
Journal article
Open
New Biological Insights Into How Deforestation in Amazonia Affects Soil Microbial Communities Using Metagenomics and Metagenome-Assembled Genomes
Creators
- 1. University of Massachusetts Amherst
- 2. University of Chicago
- 3. University of Oregon
- 4. Michigan State University
- 5. University of California, Davis
- 6. DOE Joint Genome Institute
- 7. University of São Paulo
Description
Deforestation in the Brazilian Amazon occurs at an alarming rate, which has broad effects on global greenhouse gas emissions, carbon storage, and biogeochemical cycles. In this study, soil metagenomes and metagenome-assembled genomes (MAGs) were analyzed for alterations to microbial community composition, functional groups, and putative physiology as it related to land-use change and tropical soil. A total of 28 MAGs were assembled encompassing 10 phyla, including both dominant and rare biosphere lineages. Amazon Acidobacteria subdivision 3, Melainabacteria, Microgenomates, and Parcubacteria were found exclusively in pasture soil samples, while Candidatus Rokubacteria was predominant in the adjacent rainforest soil. These shifts in relative abundance between land-use types were supported by the different putative physiologies and life strategies employed by the taxa. This research provides unique biological insights into candidate phyla in tropical soil and how deforestation may impact the carbon cycle and affect climate change.
Data availability
Contigs for all MAGs are available on figshare and NCBI BioProject PRJNA432584. The raw sequence FASTA files for the ten metagenomes are available at JGI (Supplementary Table S1).Files
fmicb-09-01635.pdf
Files
(7.7 MB)
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Additional details
Identifiers
- DOI
- 10.3389/fmicb.2018.01635
- Other
- oai:uchicago.tind.io:14829
Funding
- National Science Foundation
- DEB 14422214
- National Science Foundation
- FAPESP 446
- United States Department of Agriculture
- 2009-447 35319-05186
- United States Department of Energy
- DE-AC02-442 05CH11231