@article{TEXTUAL,
      recid = {9969},
      author = {Sherrard, Kristin M. and Cetera, Maureen and  Horne-Badovinac, Sally},
      title = {DAAM mediates the assembly of long-lived, treadmilling  stress fibers in collectively migrating epithelial cells in  <i>Drosophila</i>},
      journal = {eLife},
      address = {2021-11-23},
      number = {TEXTUAL},
      abstract = {Stress fibers (SFs) are actomyosin bundles commonly found  in individually migrating cells in culture. However,  whether and how cells use SFs to migrate in vivo or  collectively is largely unknown. Studying the collective  migration of the follicular epithelial cells in Drosophila,  we found that the SFs in these cells show a novel  treadmilling behavior that allows them to persist as the  cells migrate over multiple cell lengths. Treadmilling SFs  grow at their fronts by adding new integrin-based adhesions  and actomyosin segments over time. This causes the SFs to  have many internal adhesions along their lengths, instead  of adhesions only at the ends. The front-forming adhesions  remain stationary relative to the substrate and typically  disassemble as the cell rear approaches. By contrast, a  different type of adhesion forms at the SF’s terminus that  slides with the cell’s trailing edge as the actomyosin  ahead of it shortens. We further show that SF treadmilling  depends on cell movement and identify a developmental  switch in the formins that mediate SF assembly, with  Dishevelled-associated activator of morphogenesis acting  during migratory stages and Diaphanous acting during  postmigratory stages. We propose that treadmilling SFs keep  each cell on a linear trajectory, thereby promoting the  collective motility required for epithelial migration.},
      url = {http://knowledge.uchicago.edu/record/9969},
}