@article{TEXTUAL,
      recid = {9925},
      author = {Chakraborty, Kasturi and Leung, KaHo and Krishnan, Yamuna},
      title = {High lumenal chloride in the lysosome is critical for  lysosome function},
      journal = {eLife},
      address = {2017-07-25},
      number = {TEXTUAL},
      abstract = {Lysosomes are organelles responsible for the breakdown and  recycling of cellular machinery. Dysfunctional lysosomes  give rise to lysosomal storage disorders as well as common  neurodegenerative diseases. Here, we use a DNA-based,  fluorescent chloride reporter to measure lysosomal chloride  in Caenorhabditis elegans as well as murine and human cell  culture models of lysosomal diseases. We find that the  lysosome is highly enriched in chloride, and that chloride  reduction correlates directly with a loss in the  degradative function of the lysosome. In nematodes and  mammalian cell culture models of diverse lysosomal  disorders, where previously only lysosomal pH dysregulation  has been described, massive reduction of lumenal chloride  is observed that is ~10<sup>3</sup> fold greater than the  accompanying pH change. Reducing chloride within the  lysosome impacts Ca<sup>2+</sup> release from the lysosome  and impedes the activity of specific lysosomal enzymes  indicating a broader role for chloride in lysosomal  function.},
      url = {http://knowledge.uchicago.edu/record/9925},
}