@article{TEXTUAL,
      recid = {9783},
      author = {McGorty, Ryan J. and Currie, Christopher J. and Michel,  Jonathan and Sasanpour, Mehrzad and Gunter, Christopher and  Lindsay, K. Alice and Rust, Michael J. and Katira, Parag  and Das, Moumita and Ross, Jennifer L. and  Robertson-Anderson, Rae M.},
      title = {Kinesin and myosin motors compete to drive rich multiphase  dynamics in programmable cytoskeletal composites},
      journal = {PNAS Nexus},
      address = {2023-07-31},
      number = {TEXTUAL},
      abstract = {The cellular cytoskeleton relies on diverse populations of  motors, filaments, and binding proteins acting in concert  to enable nonequilibrium processes ranging from mitosis to  chemotaxis. The cytoskeleton's versatile reconfigurability,  programmed by interactions between its constituents, makes  it a foundational active matter platform. However, current  active matter endeavors are limited largely to single  force-generating components acting on a single  substrate—far from the composite cytoskeleton in cells.  Here, we engineer actin–microtubule (MT) composites, driven  by kinesin and myosin motors and tuned by crosslinkers, to  ballistically restructure and flow with speeds that span  three orders of magnitude depending on the composite  formulation and time relative to the onset of motor  activity. Differential dynamic microscopy analyses reveal  that kinesin and myosin compete to delay the onset of  acceleration and suppress discrete restructuring events,  while passive crosslinking of either actin or MTs has an  opposite effect. Our minimal advection–diffusion model and  spatial correlation analyses correlate these dynamics to  structure, with motor antagonism suppressing  reconfiguration and demixing, while crosslinking enhances  clustering. Despite the rich formulation space and emergent  formulation-dependent structures, the nonequilibrium  dynamics across all composites and timescales can be  organized into three classes—slow isotropic reorientation,  fast directional flow, and multimode restructuring.  Moreover, our mathematical model demonstrates that diverse  structural motifs can arise simply from the interplay  between motor-driven advection and frictional drag. These  general features of our platform facilitate applicability  to other active matter systems and shed light on diverse  ways that cytoskeletal components can cooperate or compete  to enable wide-ranging cellular processes.},
      url = {http://knowledge.uchicago.edu/record/9783},
}