000009648 001__ 9648
000009648 005__ 20240523043520.0
000009648 02470 $$ahttps://doi.org/10.3389/fpls.2023.1276472$$2doi
000009648 037__ $$bArticle
000009648 037__ $$aTEXTUAL
000009648 041__ $$aeng
000009648 245__ $$aMicrobe-associated molecular pattern recognition receptors have little effect on endophytic &lt;i&gt;Arabidopsis thaliana&lt;/i&gt; microbiome assembly in the field
000009648 269__ $$a2023-11-08
000009648 336__ $$aArticle
000009648 520__ $$aPlant microbiome structure affects plant health and productivity. A limited subset of environmental microbes successfully establishes within plant tissues, but the forces underlying this selectivity remain poorly characterized. Transmembrane pattern recognition receptors (PRRs), used by plants to detect microbe-associated molecular patterns (MAMPs), are strong candidates for achieving this selectivity because PRRs can potentially interact with many members of the microbiome. Indeed, MAMPs found in many microbial taxa, including beneficials and commensals, can instigate a robust immune response that affects microbial growth. Surprisingly, we found that MAMP-detecting PRRs have little effect on endophytic bacterial and fungal microbiome structure in the field. We compared the microbiomes of four PRR knockout lines of Arabidopsis thaliana to wild-type plants in multiple tissue types over several developmental stages and detected only subtle shifts in fungal, but not bacterial, β-diversity in one of the four PRR mutants. In one developmental stage, lore mutants had slightly altered fungal β-diversity, indicating that LORE may be involved in plant-fungal interactions in addition to its known role in detecting certain bacterial lipids. No other effects of PRRs on α-diversity, microbiome variability, within-individual homogeneity, or microbial load were found. The general lack of effect suggests that individual MAMP-detecting PRRs are not critical in shaping the endophytic plant microbiome. Rather, we suggest that MAMP-detecting PRRs must either act in concert and/or are individually maintained through pleiotropic effects or interactions with coevolved mutualists or pathogens. Although unexpected, these results offer insights into the role of MAMP-detecting PRRs in plant-microbe interactions and help direct future efforts to uncover host genetic elements that control plant microbiome assembly.
000009648 536__ $$oNational Institute of General Medical Sciences$$cT32GM007197
000009648 536__ $$oUniversity of Chicago$$aHutchinson Fund
000009648 536__ $$oEuropean Research Council$$cPATHOCOM (ID 951444)$$aSynergy Grant
000009648 536__ $$oChina Scholarship Council$$c201704910213
000009648 540__ $$a&lt;p&gt;© 2023 Oldstone-Jackson, Huang and Bergelson&lt;/p&gt; &lt;p&gt;This is an open-access article distributed under the terms of the &lt;a href="http://creativecommons.org/licenses/by/4.0/" target="_blank"&gt;Creative Commons Attribution License (CC BY)&lt;/a&gt;. The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.&lt;/p&gt;
000009648 542__ $$fCC BY
000009648 594__ $$aThe data presented in this study are deposited in the NCBI SRA repository, accession number PRJNA1015384. Scripts and commands used in data processing and analysis are available at &lt;a href="https://github.com/carolineoj/MAMPR_microbiome" target="_blank"&gt;https://github.com/carolineoj/MAMPR_microbiome&lt;/a&gt;.
000009648 6531_ $$amicrobiome
000009648 6531_ $$amicrobial diversity
000009648 6531_ $$amicrobial communities
000009648 6531_ $$aplant immunity
000009648 6531_ $$amicrobe-associated molecular pattern
000009648 6531_ $$aMAMP
000009648 6531_ $$aArabidopsis
000009648 6531_ $$afield
000009648 690__ $$aBiological Sciences Division
000009648 691__ $$aEcology and Evolution
000009648 7001_ $$aOldstone-Jackson, Caroline$$uUniversity of Chicago
000009648 7001_ $$aHuang, Feng$$uUniversity of Chicago
000009648 7001_ $$aBergelson, Joy$$uUniversity of Chicago
000009648 773__ $$tFrontiers in Plant Science
000009648 8564_ $$yArticle$$9cd7a1279-c717-40b8-9044-328242ffef75$$s3525332$$uhttps://knowledge.uchicago.edu/record/9648/files/Microbe-associated-molecular-pattern-recognition-receptors.pdf$$ePublic
000009648 8564_ $$ySupplementary material$$96f794c96-2fba-4842-9cc1-034100bf9ad0$$s1182380$$uhttps://knowledge.uchicago.edu/record/9648/files/Data-Sheet-1.pdf$$ePublic
000009648 908__ $$aI agree
000009648 909CO $$ooai:uchicago.tind.io:9648$$pGLOBAL_SET
000009648 983__ $$aArticle