@article{TEXTUAL,
      recid = {6750},
      author = {Gebhardt, Michael J. and Jacobson, Rachael K. and Shuman,  Howard A.},
      title = {Seeing red; the development of pON.mCherry, a broad-host  range constitutive expression plasmid for Gram-negative  bacteria},
      journal = {PLOS ONE},
      address = {2017-03-03},
      number = {TEXTUAL},
      abstract = {The development of plasmid-mediated gene expression  control in bacteria revolutionized the field of  bacteriology. Many of these expression control systems rely  on the addition of small molecules, generally metabolites  or non-metabolized analogs thereof, to the growth medium to  induce expression of the genes of interest. The  paradigmatic example of an expression control system is the  lac system from Escherichia coli, which typically relies on  the Ptac promoter and the Lac repressor, LacI. In many  cases, however, constitutive gene expression is desired,  and other experimental approaches require the coordinated  control of multiple genes. While multiple systems have been  developed for use in E. coli and its close relatives, the  utility and/or functionality of these tools does not always  translate to other species. For example, for the  Gram-negative pathogen, Legionella pneumophila, a causative  agent of Legionnaires’ Disease, the aforementioned Ptac  system represents the only well-established expression  control system. In order to enhance the tools available to  study bacterial gene expression in L. pneumophila, we  developed a plasmid, pON.mCherry, which confers  constitutive gene expression from a mutagenized LacI  binding site. We demonstrate that pON.mCherry neither  interferes with other plasmids harboring an intact  LacI-Ptac expression system nor alters the growth of  Legionella species during intracellular growth.  Furthermore, the broad-host range plasmid backbone of  pON.mCherry allows constitutive gene expression in a wide  variety of Gram-negative bacterial species, making  pON.mCherry a useful tool for the greater research  community.},
      url = {http://knowledge.uchicago.edu/record/6750},
}