@article{TEXTUAL,
      recid = {6257},
      author = {Christley, Scott and Shogan, Benjamin and Levine, Zoe and  Koo, Hyun and Guyton, Kristina and Owens, Sarah and  Gilbert, Jack and Zaborina, Olga and Alverdy, John C.},
      title = {Comparative genetics of <i>Enterococcus  faecalis</i> intestinal tissue isolates before and  after surgery in a rat model of colon anastomosis},
      journal = {PLOS ONE},
      address = {2020-04-28},
      number = {TEXTUAL},
      abstract = {We have recently demonstrated that collagenolytic  Enterococcus faecalis plays a key and causative role in the  pathogenesis of anastomotic leak, an uncommon but  potentially lethal complication characterized by disruption  of the intestinal wound following segmental removal of the  colon (resection) and its reconnection (anastomosis). Here  we hypothesized that comparative genetic analysis of E.  faecalis isolates present at the anastomotic wound site  before and after surgery would shed insight into the  mechanisms by which collagenolytic strains are selected for  and predominate at sites of anastomotic disruption. Whole  genome optical mapping of four pairs of isolates from rat  colonic tissue obtained following surgical resection  (herein named “pre-op” isolates) and then 6 days later from  the anastomotic site (herein named “post-op” isolates)  demonstrated that the isolates with higher collagenolytic  activity formed a distinct cluster. In order to perform  analysis at a deeper level, a single pair of E. faecalis  isolates (16A pre-op and 16A post-op) was selected for  whole genome sequencing and assembled using a hybrid  assembly algorithm. Comparative genomics demonstrated  absence of multiple gene clusters, notably a pathogenicity  island in the post-op isolate. No differences were found in  the fsr-gelE-sprE genes (EF1817-1822) responsible for  regulation and production of collagenolytic activity.  Analysis of unique genes among the 16A pre-op and post-op  isolates revealed the predominance of transporter  systems-related genes in the pre-op isolate and  phage-related and hydrolytic enzyme-encoding genes in the  post-op isolate. Despite genetic differences observed  between pre-op and post-op isolates, the precise genetic  determinants responsible for their differential expression  of collagenolytic activity remains unknown.},
      url = {http://knowledge.uchicago.edu/record/6257},
}