@article{Single-Particle:4832,
      recid = {4832},
      author = {Liu, Chang},
      title = {Structural Studies on Protein Complexes by Single-Particle  Cryogenic Electron Microscopy},
      publisher = {University of Chicago},
      school = {Ph.D.},
      address = {2022-08},
      pages = {112},
      abstract = {Single-particle cryo-electron microscopy (cryo-EM)  analysis plays an essential role in the structural  determination of protein complexes that are not suitable  for crystallization. Technological and methodological  advances have promoted the “resolution revolution”, leading  to a boom in structures determined by cryo-EM. In this  dissertation, I describe the molecular mechanisms of four  proteins by determining the structures of them and their  complexes at a near-atomic resolution (3–5 Å). These  studies demonstrate the essential roles of single-particle  cryo-EM analysis in structural biology and protein  sciences. I also describe the future development of cryo-EM  and structural biology, with advances in automated blotless  sample vitrification, real-time data processing, and more  accurate de novo protein structure prediction.},
      url = {http://knowledge.uchicago.edu/record/4832},
      doi = {https://doi.org/10.6082/uchicago.4832},
}