@article{Vandium-48-labeled:3983,
      recid = {3983},
      author = {Broder, Brittany},
      title = {Development and Kinetic Analysis of Emerging Positron  Emission Tomography Radiotracer Vandium-48-labeled Vanadyl  Acetylacetonate},
      publisher = {University of Chicago},
      school = {Ph.D.},
      address = {2022-06},
      pages = {158},
      abstract = {Vanadyl acetylacetonate (VO(acac)2) has shown promise as a  magnetic resonance imaging (MRI) agent due to its high  selectivity for cancer cells, which could facilitate  differentiation of carcinogenesis stages progressing from  inflammation to malignant tumor. This is especially vital  for types of cancer where inflammation is a recognized  component or precursor for tumorigenesis, such as  colorectal cancer (CRC) [32]. The novel positron emission  tomography (PET) radiotracer vanadium-48-labeled-VO(acac)2  (48VO(acac)2) is a positron-emitting, long-lived (t1/2 = 16  days) chelated compound that could be used for  physiological monitoring of relatively long biological  processes not feasible with other modalities or using other  radiotracers due to the high sensitivity of PET. This  radiotracer could not only provide a new method for  longitudinal studies and disease monitoring of CRC and  other cancers, but also perhaps elucidate the mechanisms of  VO(acac)2 compound accumulation in cancer cells. There are  currently no methods to synthesize, characterize, or use  this radiotracer in the literature.In this work, such  methods are developed. The first objective is to Produce  48V using a Compact Medical Cyclotron. Many facilities  focused on clinical radiotracer production, such as the IBA  18/9 Cyclone cyclotron used herein, do not have a dedicated  solid target system. However, we have modified a simple  beamstop to be used as a target holder. Theoretical  calculations and Monte Carlo simulations are done to assess  the efficacy of this system.
With the irradiated target,  the next objective is to Establish a New Procedure for the  Synthesis of 48VO(acac)2. The target is dissolved and the  48V extracted via column separation, chelated as  48VO(acac)2, and filtered. Methods are developed  iteratively for each step of the process. The compound is  characterized and synthesis is confirmed by gamma-ray  spectroscopy, high performance liquid chromatography  (HPLC), and thin layer chromatography (TLC); a new HPLC  method specific to VO(acac)2 was developed for this work  and compound characterization.
Using the synthesized  compound, the final objective is to Conduct In Vitro and In  Vivo Studies to Model the Kinetics of 48VO(acac)2. Cellular  and animal PET imaging studies using colorectal cancer cell  lines HCA-7 and HCT-116 are done to obtain cell-uptake and  time-activity curves. This data is used for kinetic  modelling to obtain a value for tumor distribution volume  ratio, a metric of how quickly the radiotracer enters and  exits the tissue from the blood.
The proposed research will  address and tackle these unexplored issues, potentially  leading
to significant advances in developing a new  radiotracer for PET imaging of cancer.},
      url = {http://knowledge.uchicago.edu/record/3983},
      doi = {https://doi.org/10.6082/uchicago.3983},
}