@article{Quantitatively:2579,
      recid = {2579},
      author = {Jani, Maulik Subhash},
      title = {NOckout: A DNA-Based Fluorescent Probe to Quantitatively  Map NOS3 Activity with Subcellular Spatial Resolution},
      publisher = {University of Chicago},
      school = {Ph.D.},
      address = {2020-08},
      pages = {93},
      abstract = {Compartmentalized nitric oxide (NO) production drives  critical signaling pathways in cells, yet there are no  methods to quantitatively image NO with sub-cellular  spatial resolution in living systems. Here, we introduce a  new DNA-based fluorescent reporter technology that maps NO  with sub-cellular resolution in live cells. It combines  small molecule NO detection chemistry with the sub-cellular  targetability of DNA based scaffold to provide quantifiable  NO maps using ratiometric imaging. We could thereby map the  activity of Nitric Oxide Synthase 3 (NOS3) which resides at  the plasma membrane and the trans-Golgi network. We find  that despite its lower abundance, the pool of NOS3 at the  plasma membrane is seven-fold more active than Golgi  associated NOS3. The ability to quantitatively map NO  dynamics with sub-cellular resolution provides the  potential to discover selective regulators of distinct NOS3  populations.},
      url = {http://knowledge.uchicago.edu/record/2579},
      doi = {https://doi.org/10.6082/uchicago.2579},
}