@article{Peritoneal:2550,
      recid = {2550},
      author = {Erickson, Steven Andrew},
      title = {A Novel B-1b Precursor Stage in the Peritoneal Cavity},
      publisher = {The University of Chicago},
      school = {Ph.D.},
      address = {2020-08},
      pages = {99},
      abstract = {B cells participate in constitutive immunological  processes to maintain host health and homeostasis. B cells  in the mucosa produce a homeostatic barrier of local  antibodies, while others take on innate-like phenotypes and  patrol bodily cavities, producing homeostatic IgM. There  are several unknown aspects of homeostatic B cell function  and ontogeny.  I primarily employed genetic approaches to  address questions about the function of mucosal antibody  and of the cellular origins of mucosal antibodies. I  developed new mouse models of mucosal antibody deficiency,  incl models in I developed robust Cas9-based gene editing  methods for the targeted introduction of rearranged  immunoglobuins to their corresponding loci and to disrupt  the immunoglobulin A locus. My system, called “Speed-Ig,”  is a rapid Cas9-based method for generating Ig knock-in  mouse lines with high on-target integration rates at both  heavy and light chain alleles. With standardized target  sites and promoter regions, Speed-Ig mice can be used for  comparative studies of B cell biology and vaccine  optimization in vivo. I used Speed-Ig to create panels of  mice with Ig pairs derived from IgA+ plasma cells, control  follicular B-2 cells, or from the innate-like cell lineages  B-1a and B-1b understand the cellular ontogeny of mucosal  antibody producing cells and innate-like subsets. IgA+  plasma cells appeared to derive from normal follicular B-2  precursors. Surprisingly, B-1b and B-2 Ig pairs drove both  B-1b and B-2 phenotypes, suggesting a previously unknown  lineage relationship between these subsets. I then  confirmed the B-1b/B-2 relationship with transcription  factor reporter lines and through adoptive cell transfer  experiments. In summary, my Ig knock-in approach  facilitated the discovery of previously unappreciated  aspect of innate-like B cell biology and revealed a novel  B-1b precursor stage present in the peritoneal cavity.},
      url = {http://knowledge.uchicago.edu/record/2550},
      doi = {https://doi.org/10.6082/uchicago.2550},
}