@article{THESIS,
      recid = {1763},
      author = {Vavra, Kevin Christopher},
      title = {Defining Myosin-10 Navigation Rules},
      publisher = {University of Chicago},
      school = {Ph.D.},
      address = {2016-08},
      number = {THESIS},
      pages = {118},
      abstract = {Myosin-10 an unconventional myosin motor that localizes to  tips of filopodia, long finger-

like projections from  cells, to help relocate its cargo proteins to the tips and  bodies of

filopodia. While it is known that myosin-10  moves along filopodial actin, how myosin-10

specifically  selects for filopodial actin tracks is currently unknown.  Three research groups

attempted to determine myosin-10’s  track selection ability using single molecule techniques  to

measure myosin-10 actin bundle selection behavior. Due  to the low dimerization affinity for

myosin-10 without a  high local motor concentration encouraged by cargo binding,  additional

nucleation domains were needed to be attached  to shortened constructs with the regulatory

cargo-binding  domains removed. These studies disagreed about myosin-10  track sensitivity

because one construct demonstrated clear  bundle selection not seen the other two constructs.

After  these studies were performed, the native myosin-10  coiled-coil structure was solved

and showed that all three  research groups had inadvertently fused an antiparallel  coiled-

coil to a parallel coiled-coil. We studied the  coiled-coils of the three previously designed

constructs  to determine the source of different behaviors between  constructs and link myosin-

10 bundle selection back to  full-length wild-type myosin-10. The bundle selective  construct,

which attempted to create a continuous  coiled-coil, forms an antiparallel oriented  coiled-coil.

Additionally this project tests the  flexibility of a single-alpha helix domain connecting  the

motor domain that binds actin to the coiled-coil to  demonstrate how rigidity and orientation

of the  coiled-coil in myosin-10 leads to actin track selection.},
      url = {http://knowledge.uchicago.edu/record/1763},
      doi = {https://doi.org/10.6082/uchicago.1763},
}