@article{TEXTUAL,
      recid = {14623},
      author = {Chandler, Talon and Guo, Min and Su, Yijun and Chen, Jiji  and Wu, Yicong and Liu, Junyu and Agashe, Atharva and  Fischer, Robert S. and Mehta, Shalin B. and Kumar, Abhishek  and Baskin, Tobias I. and Jaumouillé, Valentin and Liu,  Huafeng and Swaminathan, Vinay and Nain, Amrinder S. and  Oldenbourg, Rudolf and La Riviere, Patrick J. and Shroff,  Hari},
      title = {Volumetric imaging of the 3D orientation of cellular  structures with a polarized fluorescence light-sheet  microscope},
      journal = {PNAS},
      address = {2025-02-21},
      number = {TEXTUAL},
      abstract = {Polarized fluorescence microscopy is a valuable tool for  measuring molecular orientations in biological samples, but  techniques for recovering three-dimensional orientations  and positions of fluorescent ensembles are limited. We  report a polarized dual-view light-sheet system for  determining the diffraction-limited three-dimensional  distribution of the orientations and positions of ensembles  of fluorescent dipoles that label biological structures. We  share a set of visualization, histogram, and profiling  tools for interpreting these positions and orientations. We  model the distributions based on the polarization-dependent  efficiency of excitation and detection of emitted  fluorescence, using coarse-grained representations we call  orientation distribution functions (ODFs). We apply ODFs to  create physics-informed models of image formation with  spatio-angular point-spread and transfer functions. We use  theory and experiment to conclude that light-sheet tilting  is a necessary part of our design for recovering all  three-dimensional orientations. We use our system to extend  known two-dimensional results to three dimensions in  FM1-43-labeled giant unilamellar vesicles,  fast-scarlet-labeled cellulose in xylem cells, and  phalloidin-labeled actin in U2OS cells. Additionally, we  observe phalloidin-labeled actin in mouse fibroblasts grown  on grids of labeled nanowires and identify correlations  between local actin alignment and global cell-scale  orientation, indicating cellular coordination across length  scales.},
      url = {http://knowledge.uchicago.edu/record/14623},
}