@article{TEXTUAL,
      recid = {14562},
      author = {Miller, Samuel E. and Colman, Albert S. and Waldbauer,  Jacob R.},
      title = {Metaproteomics reveals functional partitioning and  vegetational variation among permafrost-affected Arctic  soil bacterial communities},
      journal = {Environmental Microbiology},
      address = {2023-06-05},
      number = {TEXTUAL},
      abstract = {Microbial activity in Arctic soils controls the cycling of  significant stores of organic carbon and nutrients. We  studied in situ processes in Alaskan soils using original  metaproteomic methods in order to relate important  heterotrophic functions to microbial taxa and to understand  the microbial response to Arctic greening. Major bacterial  groups show strong metabolic specialization in organic  topsoils. α-/β-/γ-Proteobacteria specialized in the  acquisition of small, soluble compounds, whereas  Acidobacteria, Actinobacteria, and other detritosphere  groups specialized in the degradation of plant-derived  polymers. α-/β-/γ-Proteobacteria dominated the expression  of transporters for common root exudates and limiting  nitrogenous compounds, supporting an ecological model of  dependence upon plants for carbon and competition with  plants for nitrogen. Detritosphere groups specialized in  distinct substrates, with Acidobacteria producing the most  enzymes for hemicellulose depolymerization. Acidobacteria  was the most active group across the three plant ecotypes  sampled—the largely nonvascular, lower biomass intertussock  and the largely vascular, higher biomass tussock and shrub.  Functional partitioning among bacterial groups was stable  between plant ecotypes, but certain functions associated  with α-/β-/γ-Proteobacteria were more strongly expressed in  higher biomass ecotypes. We show that refined metaproteomic  approaches can elucidate soil microbial ecology as well as  biogeochemical trajectories of major carbon stocks.},
      url = {http://knowledge.uchicago.edu/record/14562},
}