TY  - GEN
AB  - The Laurentian Great Lakes are a vast, interconnected freshwater system spanning strong physicochemical gradients, thus constituting a powerful natural laboratory for addressing fundamental questions about microbial ecology and evolution. We present a comparative analysis of pelagic microbial communities across all five Laurentian Great Lakes, focusing on Bacterial and Archaeal picoplankton characterized via 16S rRNA amplicon sequencing. We collected samples throughout the water column from the major basins of each lake in spring and summer over 2 years. Two oligotypes, classified as LD12 (Alphaproteobacteria) and acI-B1 (Actinobacteria), were among the most abundant in every sample. At the same time, microbial communities showed distinct patterns with depth during summer stratification. Deep hypolimnion samples were frequently dominated by a Chloroflexi oligotype that reached up to 19% relative abundance. Stratified surface communities differed between the colder, less productive upper lakes (Superior, Michigan, Huron) and warmer, more productive lower lakes (Erie, Ontario), in part due to an Actinobacteria oligotype (acI-C2) that averaged 7.7% of sequences in the lower lakes but <0.2% in the upper lakes. Together, our findings suggest that both hydrologic connectivity and local selective pressures shape microbial communities in the Great Lakes and establish a framework for future investigations.
AD  - University of Chicago
AD  - University of Wisconsin-Milwaukee
AD  - University of Chicago
AU  - Paver, Sara F.
AU  - Newton, Ryan J.
AU  - Coleman, Maureen L.
DA  - 2019-11-17
ID  - 14095
JF  - Environmental Microbiology
L1  - https://knowledge.uchicago.edu/record/14095/files/Environmental%20Microbiology%20-%202019%20-%20Paver%20-%20Microbial%20communities%20of%20the%20Laurentian%20Great%20Lakes%20reflect%20connectivity%20and.pdf
L1  - https://knowledge.uchicago.edu/record/14095/files/emi14862-sup.zip
L2  - https://knowledge.uchicago.edu/record/14095/files/Environmental%20Microbiology%20-%202019%20-%20Paver%20-%20Microbial%20communities%20of%20the%20Laurentian%20Great%20Lakes%20reflect%20connectivity%20and.pdf
L2  - https://knowledge.uchicago.edu/record/14095/files/emi14862-sup.zip
L4  - https://knowledge.uchicago.edu/record/14095/files/Environmental%20Microbiology%20-%202019%20-%20Paver%20-%20Microbial%20communities%20of%20the%20Laurentian%20Great%20Lakes%20reflect%20connectivity%20and.pdf
L4  - https://knowledge.uchicago.edu/record/14095/files/emi14862-sup.zip
LA  - eng
LK  - https://knowledge.uchicago.edu/record/14095/files/Environmental%20Microbiology%20-%202019%20-%20Paver%20-%20Microbial%20communities%20of%20the%20Laurentian%20Great%20Lakes%20reflect%20connectivity%20and.pdf
LK  - https://knowledge.uchicago.edu/record/14095/files/emi14862-sup.zip
N2  - The Laurentian Great Lakes are a vast, interconnected freshwater system spanning strong physicochemical gradients, thus constituting a powerful natural laboratory for addressing fundamental questions about microbial ecology and evolution. We present a comparative analysis of pelagic microbial communities across all five Laurentian Great Lakes, focusing on Bacterial and Archaeal picoplankton characterized via 16S rRNA amplicon sequencing. We collected samples throughout the water column from the major basins of each lake in spring and summer over 2 years. Two oligotypes, classified as LD12 (Alphaproteobacteria) and acI-B1 (Actinobacteria), were among the most abundant in every sample. At the same time, microbial communities showed distinct patterns with depth during summer stratification. Deep hypolimnion samples were frequently dominated by a Chloroflexi oligotype that reached up to 19% relative abundance. Stratified surface communities differed between the colder, less productive upper lakes (Superior, Michigan, Huron) and warmer, more productive lower lakes (Erie, Ontario), in part due to an Actinobacteria oligotype (acI-C2) that averaged 7.7% of sequences in the lower lakes but <0.2% in the upper lakes. Together, our findings suggest that both hydrologic connectivity and local selective pressures shape microbial communities in the Great Lakes and establish a framework for future investigations.
PY  - 2019-11-17
T1  - Microbial communities of the Laurentian Great Lakes reflect connectivity and local biogeochemistry
TI  - Microbial communities of the Laurentian Great Lakes reflect connectivity and local biogeochemistry
UR  - https://knowledge.uchicago.edu/record/14095/files/Environmental%20Microbiology%20-%202019%20-%20Paver%20-%20Microbial%20communities%20of%20the%20Laurentian%20Great%20Lakes%20reflect%20connectivity%20and.pdf
UR  - https://knowledge.uchicago.edu/record/14095/files/emi14862-sup.zip
Y1  - 2019-11-17
ER  -