@article{TEXTUAL,
      recid = {13530},
      author = {Jessop, Anna-Lee and Pirih, Primož and Wang, Limin and  Patel, Nipam H. and Clode, Peta L. and Schröder-Turk, Gerd  E. and Wilts, Bodo D.},
      title = {Elucidating nanostructural organization and photonic  properties of butterfly wing scales using hyperspectral  microscopy},
      journal = {Journal of The Royal Society Interface},
      address = {2024-09-11},
      number = {TEXTUAL},
      abstract = {Biophotonic nanostructures in butterfly wing scales remain  fascinating examples of biological functional materials,  with intriguing open questions with regard to formation and  evolutionary function. One particularly interesting  butterfly species, Erora opisena (Lycaenidae: Theclinae),  develops wing scales that contain three-dimensional  photonic crystals that closely resemble a single gyroid  geometry. Unlike most other gyroid-forming butterflies, E.  opisena develops discrete gyroid crystallites with a  pronounced size gradient hinting at a developmental  sequence frozen in time. Here, we present a novel  application of a hyperspectral (wavelength-resolved)  microscopy technique to investigate the ultrastructural  organization of these gyroid crystallites in dry, adult  wing scales. We show that reflectance corresponds to  crystallite size, where larger crystallites reflect green  wavelengths more intensely; this relationship could be used  to infer size from the optical signal. We further  successfully resolve the red-shifted reflectance signal  from wing scales immersed in refractive index liquids with  varying refractive index, including values similar to water  or cytosol. Such photonic crystals with lower refractive  index contrast may be similar to the hypothesized  nanostructural forms in the developing butterfly scales.  The ability to resolve these fainter signals hints at the  potential of this facile light microscopy method for in  vivo analysis of nanostructure formation in developing  butterflies.},
      url = {http://knowledge.uchicago.edu/record/13530},
}