TY  - GEN
AB  - &lt;i&gt;N&lt;/i&gt;&lt;sup&gt;6&lt;/sup&gt;-methyladenosine (m&lt;sup&gt;6&lt;/sup&gt;A) is the most abundant internal modification in mammalian messenger RNA (mRNA), constituting 0.1 %–0.4 % of total adenosine residues in the transcriptome. m&lt;sup&gt;6&lt;/sup&gt;A regulates mRNA stability and translation, pre-mRNA splicing, miRNA biogenesis, lncRNA binding, and many other physiological and pathological processes. While the majority of m&lt;sup&gt;6&lt;/sup&gt;As occur in a consensus motif of DRm&lt;sup&gt;6&lt;/sup&gt;ACH (D=A/G/U, R=A/G, H=U/A/C), the presence of such a motif does not guarantee methylation. Different RNA copies transcribed from the same gene may be methylated to varying levels. Within a single transcript, m&lt;sup&gt;6&lt;/sup&gt;As are not evenly distributed, showing an enrichment in long internal and terminal exons. These characteristics of m&lt;sup&gt;6&lt;/sup&gt;A deposition call for sequencing methods that not only pinpoint m&lt;sup&gt;6&lt;/sup&gt;A sites at base resolution, but also quantitate the abundance of methylation across different RNA copies. In this review, we summarize existing m&lt;sup&gt;6&lt;/sup&gt;A profiling methods, with an emphasis on next generation sequencing-(NGS−)based, site-specific, and quantitative methods, as well as several emerging single-cell methods.
AD  - University of Chicago
AD  - University of Chicago
AD  - University of Chicago
AU  - Ge, Ruiqi
AU  - He, Mengshu Emily
AU  - Tang, Weixin
DA  - 2024-04-15
ID  - 11554
JF  - Israel Journal of Chemistry
L1  - https://knowledge.uchicago.edu/record/11554/files/N6-methyladenosine-in-Mammalian-Messenger-RNA.pdf
L2  - https://knowledge.uchicago.edu/record/11554/files/N6-methyladenosine-in-Mammalian-Messenger-RNA.pdf
L4  - https://knowledge.uchicago.edu/record/11554/files/N6-methyladenosine-in-Mammalian-Messenger-RNA.pdf
LA  - eng
LK  - https://knowledge.uchicago.edu/record/11554/files/N6-methyladenosine-in-Mammalian-Messenger-RNA.pdf
N2  - &lt;i&gt;N&lt;/i&gt;&lt;sup&gt;6&lt;/sup&gt;-methyladenosine (m&lt;sup&gt;6&lt;/sup&gt;A) is the most abundant internal modification in mammalian messenger RNA (mRNA), constituting 0.1 %–0.4 % of total adenosine residues in the transcriptome. m&lt;sup&gt;6&lt;/sup&gt;A regulates mRNA stability and translation, pre-mRNA splicing, miRNA biogenesis, lncRNA binding, and many other physiological and pathological processes. While the majority of m&lt;sup&gt;6&lt;/sup&gt;As occur in a consensus motif of DRm&lt;sup&gt;6&lt;/sup&gt;ACH (D=A/G/U, R=A/G, H=U/A/C), the presence of such a motif does not guarantee methylation. Different RNA copies transcribed from the same gene may be methylated to varying levels. Within a single transcript, m&lt;sup&gt;6&lt;/sup&gt;As are not evenly distributed, showing an enrichment in long internal and terminal exons. These characteristics of m&lt;sup&gt;6&lt;/sup&gt;A deposition call for sequencing methods that not only pinpoint m&lt;sup&gt;6&lt;/sup&gt;A sites at base resolution, but also quantitate the abundance of methylation across different RNA copies. In this review, we summarize existing m&lt;sup&gt;6&lt;/sup&gt;A profiling methods, with an emphasis on next generation sequencing-(NGS−)based, site-specific, and quantitative methods, as well as several emerging single-cell methods.
PY  - 2024-04-15
T1  - &lt;i&gt;N&lt;/i&gt;&lt;sup&gt;6&lt;/sup&gt;-methyladenosine in Mammalian Messenger RNA: Function, Location, and Quantitation
TI  - &lt;i&gt;N&lt;/i&gt;&lt;sup&gt;6&lt;/sup&gt;-methyladenosine in Mammalian Messenger RNA: Function, Location, and Quantitation
UR  - https://knowledge.uchicago.edu/record/11554/files/N6-methyladenosine-in-Mammalian-Messenger-RNA.pdf
Y1  - 2024-04-15
ER  -