@article{TEXTUAL,
      recid = {11552},
      author = {Paul, Sneha and Arias, Mauricio A. and Wen, Li and Liao,  Susan E. and Zhang, Jiacheng and Wang, Xiaoshu and Regev,  Oded and Fei, Jingyi},
      title = {RNA molecules display distinctive organization at nuclear  speckles},
      journal = {iScience},
      address = {2024-03-27},
      number = {TEXTUAL},
      abstract = {RNA molecules often play critical roles in assisting the  formation of membraneless organelles in eukaryotic cells.  Yet, little is known about the organization of RNAs within  membraneless organelles. Here, using super-resolution  imaging and nuclear speckles as a model system, we  demonstrate that different sequence domains of RNA  transcripts exhibit differential spatial distributions  within speckles. Specifically, we image transcripts  containing a region enriched in binding motifs of  serine/arginine-rich (SR) proteins and another region  enriched in binding motifs of heterogeneous nuclear  ribonucleoproteins (hnRNPs). We show that these transcripts  localize to the outer shell of speckles, with the SR  motif-rich region localizing closer to the speckle center  relative to the hnRNP motif-rich region. Further, we  identify that this intra-speckle RNA organization is driven  by the strength of RNA-protein interactions inside and  outside speckles. Our results hint at novel functional  roles of nuclear speckles and likely other membraneless  organelles in organizing RNA substrates for biochemical  reactions.},
      url = {http://knowledge.uchicago.edu/record/11552},
}