@article{THESIS,
      recid = {11335},
      author = {Liu, Qinzhe},
      title = {Novel Approaches in DNA Methylation Analysis: Sequencing  Strategy Development and Application to Cancer Metastasis  Studies},
      publisher = {University of Chicago},
      school = {Ph.D.},
      address = {2024-03},
      number = {THESIS},
      pages = {105},
      abstract = {The study of epigenetics, particularly DNA methylation,  provides crucial insights into gene regulation and its  aberrations in diseases such as cancer. This dissertation  is anchored in the exploration of DNA methylation, a  fundamental epigenetic modification critically involved in  cellular differentiation, developmental processes, and  tumorigenesis. To advance the exploration of methylation  landscapes, we have developed a novel enzymatic-based,  bisulfite-free sequencing strategy, TT-5mC-seq. This method  achieves single-base resolution in the detection of  5-methylcytosine (5mC) modifications, markedly reducing  background noise without causing damage to the DNA. On the  other hand, we have characterized the patterns of  5-hydroxymethylcytosine (5hmC) across various human  tissues, both normal and cancerous, revealing the subtle  yet consequential shifts in the epigenetics associated with  disease states. This research provides not only new  methodologies for probing DNA methylation but also enhances  our understanding of 5hmC's potential as a biomarker for  cancer metastasis. },
      url = {http://knowledge.uchicago.edu/record/11335},
      doi = {https://doi.org/10.6082/uchicago.11335},
}