@article{THESIS,
      recid = {11330},
      author = {Satterwhite, Rebecca},
      title = {Ecological Adaptation in the Ubiquitous Plant-Pathogen  Pseudomonas syringae},
      publisher = {University of Chicago},
      school = {Ph.D.},
      address = {2024-03},
      number = {THESIS},
      pages = {126},
      abstract = {The one constant for microbial pathogens in a dynamic  natural world is change. It has been suggested that  microbial genomes harbor large numbers of standing genetic  variants because it is adaptive to have abundant raw  material on which for selection to act (McInerney et al.  2017). One type of gene that we expect to find  differentiating along with pathogen ecology are effectors.  Effectors are secreted proteins that alter the local  environment surrounding the cell, either by altering the  microbiome/microbial ecology or the host directly. Often  considered determinates of host range, effectors are an  important class of gene for the understanding of infection  outcomes. In addition to effector load, infection success  is influenced by host ecology. It remains an open question  whether strains classified into pathogenic varieties based  on host-of-isolation are actually adapted to those hosts.  With my dissertation, I ask a series of questions aimed at  elucidating the ecological adaptation of Pseudomonas  syringae. First, I perform a pangenome analysis on strains  from multiple, ecologically distinct sources and ask how  gene content delineates across groups. Next, I focused on  five strains and performed a series of controlled growth  assays and infections in common lab cultivation media as  well as the pathogen’s own, and each other’s,  hosts-of-isolation. Thus, I found evidence that local  adaptation is present, but not universal, in this  experimental system. Finally, I attempted to generate a  TnSeq dataset that would enable me to identify a  host-specific accessory genome per each of the five  pathogens. However, I was unable to generate this dataset  and instead present my best explication of my attempts in  the hopes that it will benefit future attempts. This  dissertation informs our general understanding of genome  evolution and ecological adaptation in P. syringae.},
      url = {http://knowledge.uchicago.edu/record/11330},
      doi = {https://doi.org/10.6082/uchicago.11330},
}