@article{TEXTUAL,
      recid = {10954},
      author = {Liu, Di and Shao, Yaming and Piccirilli, Joseph A. and  Weizmann, Yossi},
      title = {Structures of artificially designed discrete RNA  nanoarchitectures at near-atomic resolution},
      journal = {Science Advances},
      address = {2021-09-22},
      number = {TEXTUAL},
      abstract = {Although advances in nanotechnology have enabled the  construction of complex and functional synthetic nucleic  acid-based nanoarchitectures, high-resolution discrete  structures are lacking because of the difficulty in  obtaining good diffracting crystals. Here, we report the  design and construction of RNA nanostructures based on  homooligomerizable one-stranded tiles for x-ray  crystallographic determination. We solved three structures  to near-atomic resolution: a 2D parallelogram, a 3D  nanobracelet unexpectedly formed from an RNA designed for a  nanocage, and, eventually, a bona fide 3D nanocage designed  with the guidance of the two previous structures.  Structural details of their constituent motifs, such as  kissing loops, branched kissing loops, and T-junctions,  that resemble natural RNA motifs and resisted x-ray  determination are revealed, providing insights into those  natural motifs. This work unveils the largely unexplored  potential of crystallography in gaining high-resolution  feedback for nanoarchitectural design and suggests a route  to investigate RNA motif structures by configuring them  into nanoarchitectures.},
      url = {http://knowledge.uchicago.edu/record/10954},
}