@article{TEXTUAL,
      recid = {10904},
      author = {Wilkes, Rebecca A. and Waldbauer, Jacob and Carroll,  Austin and Nieto-Domínguez, Manuel and Parker, Darren J.  and Zhang, Lichun and Guss, Adam M. and Aristilde,  Ludmilla},
      title = {Complex regulation in a <i>Comamonas</i>  platform for diverse aromatic carbon metabolism},
      journal = {Nature Chemical Biology},
      address = {2023-02-06},
      number = {TEXTUAL},
      abstract = {Critical to a sustainable energy future are microbial  platforms that can process aromatic carbons from the  largely untapped reservoir of lignin and plastic  feedstocks. Comamonas species present promising bacterial  candidates for such platforms because they can use a range  of natural and xenobiotic aromatic compounds and often  possess innate genetic constraints that avoid competition  with sugars. However, the metabolic reactions of these  species are underexplored, and the regulatory mechanisms  are unknown. Here we identify multilevel regulation in the  conversion of lignin-related natural aromatic compounds,  4-hydroxybenzoate and vanillate, and the plastics-related  xenobiotic aromatic compound, terephthalate, in Comamonas  testosteroni KF-1. Transcription-level regulation controls  initial catabolism and cleavage, but metabolite-level  thermodynamic regulation governs fluxes in central carbon  metabolism. Quantitative <sup>13</sup>C mapping of  tricarboxylic acid cycle and cataplerotic reactions  elucidates key carbon routing not evident from enzyme  abundance changes. This scheme of transcriptional  activation coupled with metabolic fine-tuning challenges  outcome predictions during metabolic manipulations.},
      url = {http://knowledge.uchicago.edu/record/10904},
}