@article{TEXTUAL, recid = {10882}, author = {Gaudet, Mia M. and Kirchhoff, Tomas and Green, Todd and Vijai, Joseph and Korn, Joshua M. and Guiducci, Candace and Segrè, Ayellet V. and McGee, Kate and McGuffog, Lesley and Kartsonaki, Christiana and Morrison, Jonathan and Healey, Sue and Sinilnikova, Olga M. and Stoppa-Lyonnet, Dominique and Mazoyer, Sylvie and Gauthier-Villars, Marion and Sobol, Hagay and Frenay, Marc and GEMO Study Collaborators and Olopade, Olofunmilayo I}, title = {Common Genetic Variants and Modification of Penetrance of &lt;i&gt;BRCA2&lt;/i&gt;-Associated Breast Cancer}, journal = {PLOS Genetics}, address = {2010-10-28}, number = {TEXTUAL}, abstract = {<p>The considerable uncertainty regarding cancer risks associated with inherited mutations of&nbsp;<em>BRCA2</em>&nbsp;is due to unknown factors. To investigate whether common genetic variants modify penetrance for&nbsp;<em>BRCA2</em>&nbsp;mutation carriers, we undertook a two-staged genome-wide association study in&nbsp;<em>BRCA2</em>&nbsp;mutation carriers. In stage 1 using the Affymetrix 6.0 platform, 592,163 filtered SNPs genotyped were available on 899 young (&lt;40 years) affected and 804 unaffected carriers of European ancestry. Associations were evaluated using a survival-based score test adjusted for familial correlations and stratified by country of the study and&nbsp;<em>BRCA2*6174delT</em>&nbsp;mutation status. The genomic inflation factor (&lambda;) was 1.011. The stage 1 association analysis revealed multiple variants associated with breast cancer risk: 3 SNPs had p-values&lt;10<span>&minus;5</span>&nbsp;and 39 SNPs had p-values&lt;10<span>&minus;4</span>. These variants included several previously associated with sporadic breast cancer risk and two novel loci on chromosome 20 (rs311499) and chromosome 10 (rs16917302). The chromosome 10 locus was in&nbsp;<em>ZNF365</em>, which contains another variant that has recently been associated with breast cancer in an independent study of unselected cases. In stage 2, the top 85 loci from stage 1 were genotyped in 1,264 cases and 1,222 controls. Hazard ratios (HR) and 95% confidence intervals (CI) for stage 1 and 2 were combined and estimated using a retrospective likelihood approach, stratified by country of residence and the most common mutation,&nbsp;<em>BRCA2*6174delT</em>. The combined per allele HR of the minor allele for the novel loci rs16917302 was 0.75 (95% CI 0.66&ndash;0.86,&nbsp;<span class="inline-formula"><img class="inline-graphic" src="https://journals.plos.org/plosgenetics/article/file?type=thumbnail&id=10.1371/journal.pgen.1001183.e001" alt="" /></span>) and for rs311499 was 0.72 (95% CI 0.61&ndash;0.85,&nbsp;<span class="inline-formula"><img class="inline-graphic" src="https://journals.plos.org/plosgenetics/article/file?type=thumbnail&id=10.1371/journal.pgen.1001183.e002" alt="" /></span>).&nbsp;<em>FGFR2</em>&nbsp;rs2981575 had the strongest association with breast cancer risk (per allele HR = 1.28, 95% CI 1.18&ndash;1.39,&nbsp;<span class="inline-formula"><img class="inline-graphic" src="https://journals.plos.org/plosgenetics/article/file?type=thumbnail&id=10.1371/journal.pgen.1001183.e003" alt="" /></span>). These results indicate that SNPs that modify&nbsp;<em>BRCA2</em>&nbsp;penetrance identified by an agnostic approach thus far are limited to variants that also modify risk of sporadic&nbsp;<em>BRCA2</em>&nbsp;wild-type breast cancer.</p>}, url = {http://knowledge.uchicago.edu/record/10882}, }