@article{TEXTUAL,
      recid = {10881},
      author = {Dayton, Hannah and Kiss, Julie and Wei, Mian and Chauhan,  Shradha and LaMarre, Emily and Cornell, William Cole and  Morgan, Chase J. and Janakiraman, Anuradha and Min, Wei and  Tomer, Raju and Price-Whelan, Alexa and Nirody, Jasmine A.  and Dietrich, Lars E. P.},
      title = {Cellular arrangement impacts metabolic activity and  antibiotic tolerance in <i>Pseudomonas  aeruginosa</i> biofilms},
      journal = {PLOS Biology},
      address = {2024-02-01},
      number = {TEXTUAL},
      abstract = {Cells must access resources to survive, and the anatomy of  multicellular structures influences this access. In diverse  multicellular eukaryotes, resources are provided by  internal conduits that allow substances to travel more  readily through tissue than they would via diffusion.  Microbes growing in multicellular structures, called  biofilms, are also affected by differential access to  resources and we hypothesized that this is influenced by  the physical arrangement of the cells. In this study, we  examined the microanatomy of biofilms formed by the  pathogenic bacterium Pseudomonas aeruginosa and discovered  that clonal cells form striations that are packed  lengthwise across most of a mature biofilm’s depth. We  identified mutants, including those defective in pilus  function and in O-antigen attachment, that show alterations  to this lengthwise packing phenotype. Consistent with the  notion that cellular arrangement affects access to  resources within the biofilm, we found that while the wild  type shows even distribution of tested substrates across  depth, the mutants show accumulation of substrates at the  biofilm boundaries. Furthermore, we found that altered  cellular arrangement within biofilms affects the  localization of metabolic activity, the survival of  resident cells, and the susceptibility of subpopulations to  antibiotic treatment. Our observations provide insight into  cellular features that determine biofilm microanatomy, with  consequences for physiological differentiation and drug  sensitivity.},
      url = {http://knowledge.uchicago.edu/record/10881},
}