@article{TEXTUAL,
      recid = {10805},
      author = {Voelckel, Claudia and Borevitz, Justin O. and Kramer,  Elena M. and Hodges, Scott A.},
      title = {Within and between Whorls: Comparative Transcriptional  Profiling of &amp;lt;i&amp;gt;Aquilegia&amp;lt;/i&amp;gt; and  &amp;lt;i&amp;gt;Arabidopsis&amp;lt;/i&amp;gt;},
      journal = {PLOS ONE},
      address = {2010-03-23},
      number = {TEXTUAL},
      abstract = {&lt;p&gt;Background: The genus &lt;em&gt;Aquilegia&lt;/em&gt; is an emerging  model system in plant evolutionary biology predominantly  because of its wide variation in floral traits and  associated floral ecology. The anatomy of the  &lt;em&gt;Aquilegia&lt;/em&gt; flower is also very distinct. There are  two whorls of petaloid organs, the outer whorl of sepals  and the second whorl of petals that form nectar spurs, as  well as a recently evolved fifth whorl of staminodia  inserted between stamens and  carpels.&lt;/p&gt;&lt;p&gt;Methodology/Principal Findings: We designed  an oligonucleotide microarray based on EST sequences from a  mixed tissue, normalized cDNA library of an &lt;em&gt;A. formosa  x A. pubescens&lt;/em&gt; F2 population representing 17,246  unigenes. We then used this array to analyze floral gene  expression in late pre-anthesis stage floral organs from a  natural &lt;em&gt;A. formosa&lt;/em&gt; population. In particular, we  tested for gene expression patterns specific to each floral  whorl and to combinations of whorls that correspond to  traditional and modified ABC model groupings. Similar  analyses were performed on gene expression data of  &lt;em&gt;Arabidopsis thaliana&lt;/em&gt; whorls previously obtained  using the Ath1 gene chips (data available through The  Arabidopsis Information  Resource).&lt;/p&gt;&lt;p&gt;Conclusions/Significance: Our comparative  gene expression analyses suggest that 1) petaloid sepals  and petals of &lt;em&gt;A. formosa&lt;/em&gt; share gene expression  patterns more than either have organ-specific patterns, 2)  petals of &lt;em&gt;A. formosa&lt;/em&gt; and &lt;em&gt;A. thaliana&lt;/em&gt; may  be independently derived, 3) staminodia express B and C  genes similar to stamens but the staminodium genetic  program has also converged on aspects of the carpel program  and 4) staminodia have unique up-regulation of regulatory  genes and genes that have been implicated with defense  against microbial infection and herbivory. Our study also  highlights the value of comparative gene expression  profiling and the &lt;em&gt;Aquilegia&lt;/em&gt; microarray in  particular for the study of floral evolution and  ecology.&lt;/p&gt;},
      url = {http://knowledge.uchicago.edu/record/10805},
}