@article{TEXTUAL,
      recid = {10762},
      author = {Chen, Jiwang and Feng, Gang and Guo, Qiang and Wardenburg,  Juliane B. and Lin, Simon and Inoshima, Ichiro and Deaton,  Ryan and Yuan, Jason X. J. and Garcia, Joe G. N. and  Machado, Roberto F. and Otto, Michael and Wunderink,  Richard G.},
      title = {Transcriptional Events during the Recovery from MRSA Lung  Infection: A Mouse Pneumonia Model},
      journal = {PLOS ONE},
      address = {2013-08-01},
      number = {TEXTUAL},
      abstract = {<p>Community associated methicillin-resistant  <em>Staphylococcus aureus</em> (CA-MRSA) is an emerging  threat to human health throughout the world. Rodent MRSA  pneumonia models mainly focus on the early innate immune  responses to MRSA lung infection. However, the molecular  pattern and mechanisms of recovery from MRSA lung infection  are largely unknown. In this study, a sublethal mouse MRSA  pneumonia model was employed to investigate late events  during the recovery from MRSA lung infection. We compared  lung bacterial clearance, bronchoalveolar lavage fluid  (BALF) characterization, lung histology, lung cell  proliferation, lung vascular permeability and lung gene  expression profiling between days 1 and 3 post MRSA lung  infection. Compared to day 1 post infection, bacterial  colony counts, BALF total cell number and BALF protein  concentration significantly decreased at day 3 post  infection. Lung cDNA microarray analysis identified 47  significantly up-regulated and 35 down-regulated genes  (p<0.01, 1.5 fold change [up and down]). The pattern of  gene expression suggests that lung recovery is  characterized by enhanced cell division, vascularization,  wound healing and adjustment of host adaptive immune  responses. Proliferation assay by PCNA staining further  confirmed that at day 3 lungs have significantly higher  cell proliferation than at day 1. Furthermore, at day 3  lungs displayed significantly lower levels of vascular  permeability to albumin, compared to day 1. Collectively,  this data helps us elucidate the molecular mechanisms of  the recovery after MRSA lung infection.</p>},
      url = {http://knowledge.uchicago.edu/record/10762},
}