@article{TEXTUAL,
      recid = {10681},
      author = {Courson, David S. and Rock, Ronald S.},
      title = {Fast Benchtop Fabrication of Laminar Flow Chambers for  Advanced Microscopy Techniques},
      journal = {PLOS ONE},
      address = {2009-08-03},
      number = {TEXTUAL},
      abstract = {<p>Background: Fluid handling technology is acquiring an  ever more prominent place in laboratory science whether it  is in simple buffer exchange systems, perfusion chambers,  or advanced microfluidic devices. Many of these  applications remain the providence of laboratories at large  institutions with a great deal of expertise and specialized  equipment. Even with the expansion of these techniques,  limitations remain that frequently prevent the coupling of  controlled fluid flow with other technologies, such as  coupling microfluidics and high-resolution position and  force measurements by optical trapping  microscopy.</p><p>Method: Here we present a method for  fabrication of multiple-input laminar flow devices that are  optically clear [glass] on each face, chemically inert,  reusable, inexpensive, and can be fabricated on the  benchtop in approximately one hour. Further these devices  are designed to allow flow regulation by a simple gravity  method thus requiring no specialized equipment to drive  flow. Here we use these devices to perform total internal  reflection fluorescence microscopy measurements as well as  position sensitive optical trapping  experiments.</p><p>Significance: Flow chamber technology  needs to be more accessible to the general scientific  community. The method presented here is versatile and  robust. These devices use standard slides and coverslips  making them compatible with nearly all types and models of  light microscopes. These devices meet the needs of groups  doing advanced optical trapping experiments, but could also  be adapted by nearly any lab that has a function for  solution flow coupled with microscopy.</p>},
      url = {http://knowledge.uchicago.edu/record/10681},
}