@article{TEXTUAL,
      recid = {10587},
      author = {Pickrell, Joseph K. and Pai, Athma A. and Gilad, Yoav and  Pritchard, Jonathan K.},
      title = {Noisy Splicing Drives mRNA Isoform Diversity in Human  Cells},
      journal = {PLOS Genetics},
      address = {2010-12-09},
      number = {TEXTUAL},
      abstract = {<p>While the majority of multiexonic human genes show some  evidence of alternative splicing, it is unclear what  fraction of observed splice forms is functionally relevant.  In this study, we examine the extent of alternative  splicing in human cells using deep RNA sequencing and  <em>de novo</em> identification of splice junctions. We  demonstrate the existence of a large class of low abundance  isoforms, encompassing approximately 150,000 previously  unannotated splice junctions in our data. Newly-identified  splice sites show little evidence of evolutionary  conservation, suggesting that the majority are due to  erroneous splice site choice. We show that sequence motifs  involved in the recognition of exons are enriched in the  vicinity of unconserved splice sites. We estimate that the  average intron has a splicing error rate of approximately  0.7% and show that introns in highly expressed genes are  spliced more accurately, likely due to their shorter  length. These results implicate noisy splicing as an  important property of genome evolution.</p>},
      url = {http://knowledge.uchicago.edu/record/10587},
}