@article{TEXTUAL,
      recid = {10578},
      author = {Chen, Ying and Dai, Hongzheng and Chen, Sidi and Zhang,  Luoying and Long, Manyuan},
      title = {Highly Tissue Specific Expression of  &amp;lt;i&amp;gt;Sphinx&amp;lt;/i&amp;gt; Supports Its Male Courtship  Related Role in &amp;lt;i&amp;gt;Drosophila melanogaster&amp;lt;/i&amp;gt;},
      journal = {PLOS ONE},
      address = {2011-04-26},
      number = {TEXTUAL},
      abstract = {&lt;p&gt;&lt;em&gt;Sphinx&lt;/em&gt; is a lineage-specific non-coding RNA  gene involved in regulating courtship behavior in  &lt;em&gt;Drosophila melanogaster&lt;/em&gt;. The 5′ flanking region of  the gene is conserved across &lt;em&gt;Drosophila&lt;/em&gt; species,  with the proximal 300 bp being conserved out to &lt;em&gt;D.  virilis&lt;/em&gt; and a further 600 bp region being conserved  amongst the &lt;em&gt;melanogaster&lt;/em&gt; subgroup (&lt;em&gt;D.  melanogaster, D. simulans, D. sechellia, D. yakuba, and D.  erecta&lt;/em&gt;). Using a green fluorescence protein  transformation system, we demonstrated that a 253 bp region  of the highly conserved segment was sufficient to drive  &lt;em&gt;sphinx&lt;/em&gt; expression in male accessory gland. GFP  signals were also observed in brain, wing hairs and leg  bristles. An additional ∼800 bp upstream region was able to  enhance expression specifically in proboscis, suggesting  the existence of enhancer elements. Using anti-GFP  staining, we identified putative &lt;em&gt;sphinx&lt;/em&gt; expression  signal in the brain antennal lobe and inner antennocerebral  tract, suggesting that &lt;em&gt;sphinx&lt;/em&gt; might be involved in  olfactory neuron mediated regulation of male courtship  behavior. Whole genome expression profiling of the  &lt;em&gt;sphinx&lt;/em&gt; knockout mutation identified significant  up-regulated gene categories related to accessory gland  protein function and odor perception, suggesting  &lt;em&gt;sphinx&lt;/em&gt; might be a negative regulator of its target  genes.&lt;/p&gt;},
      url = {http://knowledge.uchicago.edu/record/10578},
}