@article{TEXTUAL,
      recid = {10574},
      author = {Rubin, Benjamin E. R. and Gibbons, Sean M. and Kennedy,  Suzanne and Hampton-Marcell, Jarrad and Owens, Sarah and  Gilbert, Jack A.},
      title = {Investigating the Impact of Storage Conditions on  Microbial Community Composition in Soil Samples},
      journal = {PLOS ONE},
      address = {2013-07-31},
      number = {TEXTUAL},
      abstract = {<p>Recent advances in DNA sequencing technologies have  allowed scientists to probe increasingly complex biological  systems, including the diversity of bacteria in the  environment. However, despite a multitude of recent studies  incorporating these methods, many questions regarding how  environmental samples should be collected and stored still  persist. Here, we assess the impact of different soil  storage conditions on microbial community composition using  Illumina-based 16S rRNA V4 amplicon sequencing. Both  storage time and temperature affected bacterial community  composition and structure. Frozen samples maintained the  highest alpha diversity and differed least in beta  diversity, suggesting the utility of cold storage for  maintaining consistent communities. Samples stored for  intermediate times (three and seven days) had both the  highest alpha diversity and the largest differences in  overall beta diversity, showing the degree of community  change after sample collection. These divergences  notwithstanding, differences in neither storage time nor  storage temperature substantially altered overall  communities relative to more than 500 previously examined  soil samples. These results systematically support previous  studies and stress the importance of methodological  consistency for accurate characterization and comparison of  soil microbiological assemblages.</p>},
      url = {http://knowledge.uchicago.edu/record/10574},
}