@article{TEXTUAL,
      recid = {10401},
      author = {Qin, Jie and Li, Wei-Qiang and Zhang, Li and Chen, Fei and  Liang, Wen-Hua and Mao, Frank Fuxiang and Zhang, Xiu-Ming  and Lahn, Bruce T. and Yu, Wei-Hua and Xiang, Andy Peng},
      title = {A Stem Cell-Based Tool for Small Molecule Screening in  Adipogenesis},
      journal = {PLOS ONE},
      address = {2010-09-27},
      number = {TEXTUAL},
      abstract = {<p>Techniques for small molecule screening are widely used  in biological mechanism study and drug discovery. Here, we  reported a novel adipocyte differentiation assay for small  molecule selection, based on human mesenchymal stem cells  (hMSCs) transduced with fluorescence reporter gene driven  by adipogenic specific promoter - adipocyte Protein 2 (aP2;  also namely Fatty Acid Binding Protein 4, FABP4). During  normal adipogenic induction as well as adipogenic  inhibition by Ly294002, we confirmed that the intensity of  green fluorescence protein corresponded well to the  expression level of aP2 gene. Furthermore, this variation  of green fluorescence protein intensity can be read simply  through fluorescence spectrophotometer. By testing another  two small molecules in adipogenesis –Troglitazone and  CHIR99021, we proved that this is a simple and sensitive  method, which could be applied in adipocyte biology, drug  discovery and toxicological study in the future.</p>},
      url = {http://knowledge.uchicago.edu/record/10401},
}