@article{TEXTUAL,
      recid = {10324},
      author = {Nègre, Nicolas and Brown, Christopher D. and Shah, Parantu  K. and Kheradpour, Pouya and Morrison, Carolyn A. and  Henikoff, Jorja G. and Feng, Xin and Ahmad, Kami and  Russell, Steven and White, Robert A. H. and Stein, Lincoln  and Henikoff, Steven and Kellis, Manolis and White, Kevin  P.},
      title = {A Comprehensive Map of Insulator Elements for the  <i>Drosophila</i> Genome},
      journal = {PLOS Genetics},
      address = {2010-01-15},
      number = {TEXTUAL},
      abstract = {<p>Insulators are DNA sequences that control the  interactions among genomic regulatory elements and act as  chromatin boundaries. A thorough understanding of their  location and function is necessary to address the  complexities of metazoan gene regulation. We studied by  ChIP–chip the genome-wide binding sites of 6  insulator-associated proteins—dCTCF, CP190, BEAF-32,  Su(Hw), Mod(mdg4), and GAF—to obtain the first  comprehensive map of insulator elements in  <em>Drosophila</em> embryos. We identify over 14,000  putative insulators, including all classically defined  insulators. We find two major classes of insulators defined  by dCTCF/CP190/BEAF-32 and Su(Hw), respectively.  Distributional analyses of insulators revealed that  particular sub-classes of insulator elements are excluded  between <em>cis</em>-regulatory elements and their target  promoters; divide differentially expressed, alternative,  and divergent promoters; act as chromatin boundaries; are  associated with chromosomal breakpoints among species; and  are embedded within active chromatin domains. Together,  these results provide a map demarcating the boundaries of  gene regulatory units and a framework for understanding  insulator function during the development and evolution of  <em>Drosophila</em>.</p>},
      url = {http://knowledge.uchicago.edu/record/10324},
}